Teacher Training (Antibiotic Bioprospecting)
This teacher training course was developed in collaboration with SOC, Dr. Adrian Pinto’s lab at the University of Costa Rica (UCR), Dr. Cameron Curry’s lab at the University of Wisconsin - Madison (UW): Dr. Gabriel Vargas, Ibrahim Zuñiga and Caitlin Carlson. In this course, teachers will learn how to run a bioprospecting research lab at their school. Specifically teachers will:
- isolate bacteria strains (Actinomycetes) from insect samples,
- establish isolate morphologies and other bioassay-determined characteristics,
- co-culture isolates for antimicrobial inhibition against antagonists
- use polymerase chain reaction (PCR) and electrophoresis to amplify and separate isolated genetic material by gene length,
- analyze 16S rRNA genetic sequence results to establish likely phylogenetic ancestry using MOLE-BLAST and MEGA, and
- learn how full genetic sequencing and antiSMASH identifies biosynthetic gene clusters (BGCs) that encode novel pathways for secondary metabolites with potential medical applications.
Training Workshop Details
Dates: March 4-7, 2023
Location: Augustana University, South Dakota
Instructor: Dr. Gabriel Vargas (UW – Madison)
Recommended hotel for out of town participants: SpringHill Suites by Marriott Sioux Falls: 605-444-4200 4304 W Empire Pl, Sioux Falls, SD, 57106
Class begins on Day 1 at 9 am. Daily schedule is 9-12, 1-4 pm (except last day, ends at 3 pm)
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- Seeds of Change
- 1680 Shadywood Rd,
- Wayzata, MN 55391